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They also showed the possibility to couple with SEC for the simultaneous identification of copolymer composition and molecular weight distributions. Sauzedde and Hunkeler tested the effect of molecular weight and composition in LCCC retention with two series of poly(S-co-MMA) [165]. The first series varies with respect to the chemical composition in the copolymer, at a constant molar mass, while the second has molecular weight variation at a fixed composition. The copolymers were fractionated using SEC and LCCC.

For multiblock copolymers it becomes more complicated. However, it is obvious that the analysis of block copolymers is simpler than random copolymers that can be regarded as multiblock copolymers with numerous block sizes connected in a random sequence. Major effort has been made on LCCC separation at the critical condition of one block. It is based on the assumption that a block can be made “chromatographically invisible” at its critical condition and the retention of the block copolymer is determined solely by the other block that is not under critical condition [55, 128, 129].

17 A LCCC chromatograms of PtBMA-b-PMMA. 3 kg/ mol) at the critical condition of PtBMA. Column: two Nucleosil C18; 250¥4 mm; 300 Å and 1,000 Å. 5, w/w). B LCCC chromatograms of PMMA-block-PtBMA. 6, 108, 167 kg/mol) at the critical condition of PMMA. Column: two Nucleosil; 150¥4 mm, 100 Å and 250¥4 mm, 300 Å. Eluent: THF/n-hexane (82/18, w/w). Reproduced from [44] with permission Recently Falkenhagen et al. and Lee et al. reported rather contradictory results on LCCC studies with custom-made sets of block copolymers.

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Liquid Chromatography Ftir Microspectroscopy, Microwave Assisted Synthesis by Bhargava R.

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